Printable gelatin, alginate and boron nitride nanotubes hydrogel-based ink for 3D bioprinting and tissue engineering applications

Hydrogels comprised of alginate and gelatin are potential biomaterials for extrusion-based bioprinting. However, existing polymeric inks inadequate in printability, shape fidelity mechanical properties that desire to produce scaffold using 3D Here, a novel printable hydrogel-based ink solution gelatin-alginate (GA) reinforced by boron nitride nanotubes (BNNTs) was developed bioprinting tissue engineering applications. The solutions were tested printing accuracy, compressive behaviour, while the printed scaffolds their viability human embryonic kidney cells (HEK293T). Printability tests showed strand thicknesses decreased with increasing concentration BNNTs GA solutions. Similarly, accuracy increased (>90%) loading BNNTs. swelling rate higher than scaffolds. Mechanical measured compression testing. Increasing resulted load stress. Also, quantitative analysis cell revealed slight effect on toxicity doping Hence, relevant data indicated it could be applications through processes.